Brain organoid systems
Midbrain organoids are 3D in vitro models that recapitulate the cellular features of the human midbrain. They can be generated from somatic samples taken from any healthy individual or PD patient.
Which cells are in midbrain organoids?
Neurons
The main transmitters of information in the nervous system. We can identify them with a TUJ1 marker (red).
Dopaminergic neurons
This subtype of neurons degenerates in Parkinson’s Disease, and they are especially abundant in a region called the midbrain. We can identify them with a TH marker (white).
Astrocytes
Astrocytes are star-shaped cells support others - such as neurons - in the brain. We can identify them with a GFAP marker (green).
… Many other cell types can be found in midbrain organoids, such as progenitor cells, stem cells, other neuronal subtypes, and microglia..
Microglia (green) identified by IBA1 marker presence in organoids (left) and 2D (right).
Parkinson's disease - Research and Development
About our Model
MIDBRAIN ORGANOIDS
Organoids contain a high amount of dopaminergic neurons and replicate one of the main hallmarks of PD, dopaminergic neuron loss.
FURTHER DEVELOPMENT
They can be enriched with endothelial cells and microglia, and they show neuroinflammation phenotypes.
DRUG DISCOVERY
We specialize in compound screening and drug discovery.
Our project pipeline
Our services include project design and different phases of experimental procedures and analysis.
They are fully customizable and adapted to your project needs.
Organoid generation
Project design, choice of cellular target, and culture timelines (organoid maturation status). Generation of midbrain organoids.
Compound treatment
Project design: choice of drug concentrations and treatment duration. Treatment of midbrain organoids with drug candidates.
Experimental analysis
Wet lab experiments based on the project needs (neuronal phenotypes, autophagy, mitochondrial phenotypes, neuroinflammation, etc ).
Data analysis
Complete analysis of the observed results accompanied by a discussion.
IP
Monzel A.S., Bolognin S., Schwamborn J.C. (2016) Means and methods for generating midbrain organoids: US11976296B2
